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How To Make Karo Liquid Culture.

Karo LC Recipe

Karo syrup is a popular choice for making liquid culture broth for mushroom cultivation and mycology. It is a thick, sweet syrup that is made from corn syrup and is widely available in many parts of the world. 

The Karo LC recipe produces a clear solution which allows the mycelium to be easily observed while it grows, a feature often sought after by mycologists. 

Karo Liquid Culture tek is easy to sterilize and is a good source of nutrients for mycelium, making it an ideal choice for a LC recipe. 

To ensure purity of the culture before inoculation you will need to sterilize it, so if you don't have a pressure cooker checkout my post on the best pressure cookers for mushroom cultivation.

Whether you are an experienced mycologist or just getting started in the field, this recipe is a simple and effective way to cultivate a wide variety of mycelium in the laboratory or at home.

In this guide, we'll walk you through the ingredients and materials needed, as well as step-by-step instructions for making Karo Liquid Culture.

Karo Liquid Culture Recipe

The recipe for KARO liquid culture generally consists of a 4% ratio of karo-to-water.

A teaspoon of Karo weighs around 4 grams, so it is possible to do 1 teaspoon per 100ml of water.

However, it is always best to use a scale to accurately measure ingredients whenever possible.

The Karo LC recipe is outlined in the table below: 

Water Karo Ratio (% Karo)
100ml 4g 4%
200ml 8g 4%
300ml 12g 4%
400ml 16g 4%
500ml 20g 4%

Step-By-Step Instructions For Making Karo Liquid Culture.

The basic steps are outlined below:

  1. Mix 4% Karo into hot water and stir.
  2. Sterilize the Karo/Water mixture in a jar
  3. Inoculate jar of mixture with mycelium
  4. Wait for the mycelium to grow.

Ingredients and Materials Needed:

Step 1 – Create Karo / Water Mixture.

  • I measure out the desired amount of KAro and water. For every 100ml of water, you will need 4g of KAro. I will be using 16g of Karo and 400ml of water.
  • Mix the karo and water together until the Karo is fully dissolved in the mixture.
  • Place a stirring rod inside the mixture (optional).
  • Place the lid on the jar.
  • Create a small hole in the lid using a screwdriver or similar.
  • Place two sheets of micropore tape over this hole.
  • Wrap the lid in tinfoil and secure with an elastic band.

Step 2 – Sterilize Jars.

  • I place my jars into my pressure cooker and sterilize for 30-40 minutes at 15psi. I will be using my presto 23 Qt pressure cooker for this.
  • Allow the pressure cooker to cool for several hours before continuing to the next step. I usually leave my cooker overnight just to be sure.

Step 3 – Inoculate Karo/Water Mixture.

  • Remove jars from the pressure cooker.
  • Wipe the lid of each jar with alcohol wipes.
  • Shake the needle to spread out the mycelium.
  • Flame sterilize the needle until it is glowing red.
  • Inject 1cc of liquid culture into each jar using the flame sterilized needle.
  • Cover the injection site with micropore tape, I do this as I'm pulling the needle out.
  • Place the jars in an incubation area with a temperature range of 18-20°C (64-68°F) for optimal growth.

Step 4 – Wait.

Generally colonization can take around 14 days.

Place your jar on the stirring plate and wait for it to colonize, if you don't have a stirring plate then just give it a whirl around once a day being careful not to hit the lid.

Advantages of using Karo for Liquid Culture.

There are several benefits to using Karo syrup as a medium for making liquid culture broth in mycology:

  1. Karo syrup is a simple, inexpensive, and readily available source of nutrients for mycelium.
  2. It is easy to sterilize and has a long shelf life, making it convenient to use in a laboratory setting.
  3. Karo syrup provides a consistent, nutrient-rich environment for the growth of mycelium, which can help improve the success rate of a liquid culture.
  4. Karo syrup is also a good source of energy for mycelium, which can help promote healthy growth and reproduction.
  5. Karo is a clear liquid which means a mycologist can observe its growth and spot contamination easily.
  6. Finally, Karo syrup is less viscous than other types of sugar, making it easier to inject into a jar or petri dish.

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